Anti-CD63 Antibody Picoband®

About this product

Boster Bio Anti-CD63 Antibody Picoband® catalog # PB9250. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9250

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD63 antigen is a protein that in humans is encoded by the CD63 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. It is mapped to 12q13.2. CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression.

Synonyms

CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD63; CD63; MLA1, TSPAN30

Gene Name

CD63

Gene ID

967

UniProt

P08962

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CD63 recombinant protein (Position: E97-M238) . Human CD63 shares 74% and 73% amino acid (aa) sequence identity with mouse and rat CD63, respectively.

Clonality

Polyclonal

Tissue Specificity

Detected in platelets (at protein level) . Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. .

Applications

IHC, WB

Field of Research

Cancer, Cardiovascular, Cell Adhesion, Cell Type Markers, Immunology, Invasion/Microenvironment, Platelets, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. .

References & Citations

1. Entrez Gene: CD63 CD63 molecule 2. Gwynn, B., Eicher, E. M., Peters, L. L. Genetic localization of Cd63, a member of the transmembrane 4 superfamily, reveals two distinct loci in the mouse genome. Genomics 35: 389-391, 1996.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

25637 MW

Observed Molecular Weight

30-60 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

CD63 antigen

Subcellular Location

Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosome, multivesicular body. Melanosome. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies.

Protein Name

CD63 antigen

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.