- Catalog number
- PB9251
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
- Synonyms
- T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86; CD86; CD28LG2
- Gene Name
- CD86
- Gene ID
- 942
- UniProt
- P42081
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- E.coli-derived human CD86 recombinant protein (Position: A24-F329) . Human CD86 shares 50% amino acid (aa) sequence identity with mouse CD86.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed by activated B-lymphocytes and monocytes.
- Applications
- IHC, WB
- Field of Research
- Adaptive Immunity, Cell Type Markers, Hematopoietic Progenitors, Host-Virus Interaction, Immunology, Interspecies Interaction, Lymphoid, Microbiology, Myeloid, Stem Cells, T Cells
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
- References & Citations
- 1. Chen C, Gault A, Shen L, Nabavi N (May 1994) . Molecular cloning and expression of early T cell costimulatory molecule-1 and its characterization as B7-2 molecule. J. Immunol. 152 (10) : 4929–36. 2. Reeves, R. H., Patch, D., Sharpe, A. H., Borriello, F., Freeman, G. J., Edelhoff, S., Disteche, C. The costimulatory genes Cd80 and Cd86 are linked on mouse chromosome 16 and human chromosome 3. Mammalian Genome 8: 581-582, 1997. 3. Shah, R., Banks, K., Patel, A., Dogra, S., Terrell, R., Powers, P. A., Fenton, C., Dinauer, C. A., Tuttle, R. M., Francis, G. L. Intense expression of the B7-2 antigen presentation coactivator is an unfavorable prognostic indicator for differentiated thyroid carcinoma of children and adolescents. J. Clin. Endocr. Metab. 87: 4391-4397, 2002.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 37682 MW
- Observed Molecular Weight
- 60-80 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- T-lymphocyte activation antigen CD86
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
- Protein Name
- T-lymphocyte activation antigen CD86
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
