Anti-Prostate Specific Antigen/KLK3 Antibody Picoband®

About this product

Boster Bio Anti-Prostate Specific Antigen/KLK3 Antibody Picoband® catalog # PB9259. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9259

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme encoded in humans by the KLK3 gene. KLK3 is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. This gene is mapped to 19q13.33. KLK3 is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. KLK3 is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm. It is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia.

Synonyms

Prostate-specific antigen; PSA;3.4.21.77; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase; KLK3; APS

Gene Name

KLK3

Gene ID

354

UniProt

P07288

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PSA recombinant protein (Position: I25-P261) . Human PSA shares 54% amino acid (aa) sequence identity with rat PSA.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level) . Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells. .

Applications

IHC, WB

Field of Research

Cancer, Cell Adhesion Proteins, Cell Type Markers, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Neuroscience, Signal Transduction, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

References & Citations

1. Balk SP, Ko YJ, Bubley GJ (January 2003) . Biology of prostate-specific antigen. J. Clin. Oncol. 21 (2) : 383–91. 2. Catalona, W. J.; Richie, J. P.; Ahmann, F. R.; Hudson, M. A.; Scardino, P. T.; Flanigan, R. C.; Dekernion, J. B.; Ratliff, T. L.; Kavoussi, L. R.; Dalkin, B. L. (1994) . Comparison of digital rectal examination and serum prostate specific antigen in the early detection of prostate cancer: Results of a multicenter clinical trial of 6,630 men. The Journal of urology 151 (5) : 1283–1290. 3. Lilja H (November 2003) . Biology of prostate-specific antigen. Urology 62 (5 Suppl 1) : 27–33. doi:10.1016/S0090-4295 (03) 00775-1.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

28741 MW

Observed Molecular Weight

29 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Prostate-specific antigen

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein Name

Prostate-specific antigen

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.