Anti-Menin/MEN1 Antibody Picoband®

About this product

Boster Bio Anti-Menin/MEN1 Antibody Picoband® catalog # PB9263. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9263

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The MEN1 gene encodes menin, a nuclear scaffold protein that regulates gene transcription by coordinating chromatin remodeling. It is mapped to 11q13.1. MEN1 is considered to act as a tumor suppressor gene. It has been found that that MEN1 inactivation by antisense RNA antagonizes transforming growth factor-beta-mediated cell growth inhibition. Overexpression of MEN1 in an inducible cell culture system downregulated the proximal promoter. In vitro studies have shown that MEN1 is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD. What’s more, MEN1 was essential to maintain MLL-associated myeloid transformation.

Synonyms

Menin; MEN1; SCG2

Gene Name

MEN1

Gene ID

4221

UniProt

O00255

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Menin recombinant protein (Position: P301-L615) . Human Menin shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Menin, respectively.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

WB

Field of Research

Cancer Susceptibility,2339, Epigenetics and Nuclear Signaling, Transcription, Tumor Suppressors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4) . Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity) . May be involved in DNA repair. .

References & Citations

1. Canaff, L., Vanbellinghen, J.-F., Kaji, H., Goltzman, D., Hendy, G. N. Impaired transforming growth factor-beta (TGF-beta) transcriptional activity and cell proliferation control of a menin in-frame deletion mutant associated with multiple endocrine neoplasia type 1 (MEN1) . J. Biol. Chem. 287: 8584-8597, 2012. 2. Kaji, H., Canaff, L., Lebrun, J.-J., Goltzman, D., Hendy, G. N. Inactivation of menin, a Smad3-interacting protein, blocks transforming growth factor type beta signaling. Proc. Nat. Acad. Sci. 98: 3837-3842, 2001. 3. Zablewska, B., Bylund, L., Mandic, S. A., Fromaget, M., Gaudray, P., Weber, G. Transcription regulation of the multiple endocrine neoplasia type 1 gene in human and mouse. J. Clin. Endocr. Metab. 88: 3845-3851, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68023 MW

Observed Molecular Weight

68 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Menin

Subcellular Location

Nucleus . Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation.

Protein Name

Menin

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.01 mg NaN3.