Anti-ABCB4 Antibody Picoband®

About this product

Boster Bio Anti-ABCB4 Antibody Picoband® catalog # PB9275. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9275

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Adenosine triphosphate-binding cassette subfamily B, member 4 (ABCB4), also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. The ABCB4 gene contains 28 exons and 27 of these contain coding sequences for the two homologous halves of the protein that correlate with functional domains. ABCB4 gene mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.

Synonyms

Multidrug resistance protein 3;3.6.3.44; ATP-binding cassette sub-family B member 4; P-glycoprotein 3; ABCB4; MDR3, PGY3

Gene Name

ABCB4

Gene ID

5244

UniProt

P21439

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human ABCB4 recombinant protein (Position: A601-A720) . Human ABCB4 shares 79% amino acid (aa) sequence identity with both mouse and rat ABCB4.

Clonality

Polyclonal

Tissue Specificity

Plasma. Expressed in liver. .

Applications

Flow Cytometry, IHC, WB

Field of Research

ATPases, Cancer, Lipid Metabolism, Metabolism, Nuclear Import/Export, Plasma Membrane, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

References & Citations

1. Lincke, C. R., Smit, J. J., van der Velde Koerts, T., Borst, P. Structure of the human MDR3 gene and physical mapping of the human MDR locus. J. Biol. Chem. 266: 5303-5310, 1991. 2. Rosmorduc, O., Hermelin, B., Poupon, R. MDR3 gene defect in adults with symptomatic intrahepatic and gallbladder cholesterol cholelithiasis. Gastroenterology 120: 1459-1467, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

141523 MW

Observed Molecular Weight

150 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Multidrug resistance protein 3

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Protein Name

Phosphatidylcholine translocator ABCB4

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.