- Catalog number
- RP1054
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- SERCA2, also called ATP2A2 or ATP2B, encodes one of the SERCA Ca (2+) -ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. They are closely related to the plasma membrane Ca (2+) -ATPases, or PMCAs. SERCA2 belongs to the large family of P-type cation pumps that couple ATP hydrolysis with cation transport across membranes. The SERCA2 gene is mapped to 12q24.11. SERCA2 was expressed in all specimens, with pronounced expression in the subnuclear aspect of basal epidermal keratinocytes. There was variable suprabasal expression. SERCA2 expression was also observed in the infundibulum and outer root sheath of hair follicles; germinative and mature cells of sebaceous glands; secretory coil and duct of eccrine glands; apocrine gland cells; and arrector pili muscle. In Darier disease skin, strong SERCA2 positivity was detected in the basal, suprabasal, and acantholytic lesional cells.
- Synonyms
- Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2; SR Ca (2+) -ATPase 2;3.6.3.8; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca (2+) ATPase; ATP2A2; ATP2B
- Gene Name
- ATP2A1
- Gene ID
- 488
- UniProt
- P16615
- Host
- Rabbit
- Reactivity
- Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human SERCA2 ATPase, identical to the related mouse and rat sequences.
- Clonality
- Polyclonal
- Tissue Specificity
- Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart. .
- Applications
- IHC, WB
- Field of Research
- ATPases, Calcium Channels, Calcium Signaling, Cancer, Cardiovascular, Cofactors, Vitamins/Minerals, Contractility, Metabolism, Pathways and Processes, Plasma Membrane, Signal Transduction, Signaling Pathway, Vitamins/Minerals
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. .
- References & Citations
- 1. Ahn, W., Lee, M. G., Kim, K. H., Muallem, S. Multiple effects of SERCA2b mutations associated with Darier's disease. J. Biol. Chem. 278: 20795-20801, 2003. 2. Berk, D. R., Taube, J. M., Bruckner, A. L., Lane, A. T. A sporadic patient with acrokeratosis verruciformis of Hopf and a novel ATP2A2 mutation. (Letter) Brit. J. Derm. 163: 653-654, 2010. 3. Chao, S.-C., Yang, M.-H., Lee, J. Y.-Y. Mutation analysis of the ATP2A2 gene in Taiwanese patients with Darier's disease. Brit. J. Derm. 146: 958-963, 2002.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 114757 MW
- Observed Molecular Weight
- 115 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
- Subcellular Location
- Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.
- Sequence Similarities
- Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
- Protein Name
- Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
