Anti-SERCA3 ATPase/ATP2A3 Antibody Picoband®

About this product

Boster Bio Anti-SERCA3 ATPase/ATP2A3 Antibody catalog # RP1055. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1055

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, also known as SERCA3, is anenzyme that in humans is encoded by the ATP2A3 gene. It is mapped to 17p13.2. This gene encodes one of the SERCA Ca2+-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of cells. ATP2A3 expression was originally described as non-muscular, but was recently observed in cardiomyocyte. What’s more, the expression of ATP2A3 was significantly reduced or lost in colon carcinomas compared with normal colonic epithelial cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction.

Synonyms

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3; SERCA3; SR Ca (2+) -ATPase 3;3.6.3.8; Calcium pump 3; ATP2A3

Gene Name

ATP2A3

Gene ID

489

UniProt

Q93084

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ATP2A3, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.

Clonality

Polyclonal

Tissue Specificity

Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues. Expressed in cell lineages of hematopoietic, epithelial, or embryonic origin and also expressed in several cancer cell lines. .

Applications

IHC, WB

Field of Research

ATPases, Calcium Signaling, Cancer, Metabolism, Plasma Membrane, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. .

References & Citations

1. Dode L, Wuytack F, Kools PF, Baba-Aissa F, Raeymaekers L, Brike F, van de Ven WJ, Casteels R (Nov 1996) . 2. Gelebart, P., Kovacs, T., Brouland, J.-P., van Gorp, R., Grossmann, J., Rivard, N., Panis, Y., Martin, V., Bredoux, R., Enouf, J., Papp, B. Expression of endomembrane calcium pumps in colon and gastric cancer cells: induction of SERCA3 expression during differentiation. J. Biol. Chem. 277: 26310-26320, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

113977 MW

Observed Molecular Weight

114 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

Subcellular Location

Nucleus membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Sarcoplasmic reticulum membrane ; Multi-pass membrane protein .

Sequence Similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

Protein Name

Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.