Anti-Occludin/OCLN Antibody Picoband®

About this product

Boster Bio Anti-Occludin/OCLN Antibody catalog # RP1057. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1057

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.

Synonyms

Occludin; OCLN

Gene Name

OCLN

Gene ID

100506658

UniProt

Q16625

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Occludin recombinant protein (Position: N373-T522) . Human Occludin shares 89% amino acid (aa) sequence identity with both mouse and rat Occludin.

Clonality

Polyclonal

Tissue Specificity

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

Applications

IHC, WB

Field of Research

Cell Adhesion, Cytoskeleton/ECM, Signal Transduction, Tight Junctions

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. .

References & Citations

1. Ando-Akatsuka, Y., Saitou, M., Hirase, T., Kishi, M., Sakakibara, A., Itoh, M., Yonemura, S., Furuse, M., Tsukita, S. Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues. J. Cell Biol. 133: 43-47, 1996. 2. Barrios-Rodiles, M., Brown, K. R., Ozdamar, B., Bose, R., Liu, Z., Donovan, R. S., Shinjo, F., Liu, Y., Dembowy, J., Taylor, I. W., Luga, V., Przulj, N., Robinson, M., Suzuki, H., Hayashizaki, Y., Jurisica, I., Wrana, J. L. High-throughput mapping of a dynamic signaling network in mammalian cells. Science 307: 1621-1625, 2005. 3. Du, D., Xu, F., Yu, L., Zhang, C., Lu, X., Yuan, H., Huang, Q., Zhang, F., Bao, H., Jia, L., Wu, X., Zhu, X., Zhang, X., Zhang, Z., Chen, Z. The tight junction protein, occludin, regulates the ional migration of epithelial cells. Dev. Cell 18: 52-63, 2010.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

59144 MW

Observed Molecular Weight

59 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Occludin

Subcellular Location

Membrane; Multi-pass membrane protein. Cell junction, tight junction.

Sequence Similarities

Belongs to the ELL/occludin family.

Protein Name

Occludin

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.