Anti-Sex Hormone Binding Globulin/SHBG Antibody Picoband®

About this product

Boster Bio Anti-Sex Hormone Binding Globulin/SHBG Antibody Picoband® catalog # PB9326. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9326

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sex hormone-binding globulin (SHBG) is a glycoprotein that binds to the sex hormones, androgen and estrogen. SHBG is produced mostly by the liver and is released into the bloodstream. Other sites that produce SHBG include the brain, uterus, testes, and placenta. SHBG has both enhancing and inhibiting hormonal influences. It decreases with high levels of insulin, growth hormone, insulin-like growth factor 1 (IGF-1), androgens, prolactin and transcortin. High estrogen, andthyroxine cause it to increase. It has been found that the monosaccharides glucose and fructose reduced human SHBG production by hepatocytes, irrespective of the presence of insulin, by downregulation of hepatocyte nuclear factor-4A and replacement of HNF4A by COUP-TFI at a cis-element within the human SHBG promoter, coincident with repression of its transcriptional activity.

Synonyms

Sex hormone-binding globulin; SHBG; Sex steroid-binding protein; SBP; Testis-specific androgen-binding protein; ABP; Testosterone-estradiol-binding globulin; TeBG; Testosterone-estrogen-binding globulin; SHBG

Gene Name

SHBG

Gene ID

6462

UniProt

P04278

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SHBG recombinant protein (Position: L30-A210) . Human SHBG shares 72% amino acid (aa) sequence identity with both mouse and rat SHBG.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 and isoform 2 are present in liver and testis.

Applications

WB

Field of Research

Cancer, Growth Factors/Hormones, Lipid Metabolism, Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.

References & Citations

1. Hammond GL (2011) . Diverse Roles for Sex Hormone-Binding Globulin in Reproduction. Biology of Reproduction 85 (3) : 431–441. 2. Selva, D. M., Hogeveen, K. N., Innis, S. M., Hammond, G. L. Monosaccharide-induced lipogenesis regulates the human hepatic sex hormone-binding globulin gene. J. Clin. Invest. 117: 3979-3987, 2007. 3. Simó R, Barbosa-Desongles A, Hernandez C, Selva DM (November 2012) . IL1β down-regulation of sex hormone-binding globulin production by decreasing HNF-4α via MEK-1/2 and JNK MAPK pathways. Mol. Endocrinol. 26 (11) : 1917–27.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

43779 MW

Observed Molecular Weight

70 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Sex hormone-binding globulin

Subcellular Location

Secreted . In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis. .

Sequence Similarities

Contains 2 laminin G-like domains.

Protein Name

Sex hormone-binding globulin

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.