Anti-Dystrophin/DMD Antibody Picoband®

About this product

Boster Bio Anti-Dystrophin/DMD Antibody Picoband® catalog # PB9276. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9276

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dystrophin, also known as DMD, is a rod-shaped cytoplasmic protein, and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. It is mapped to Xp21.2-p21.1. This complex is variously known as the costamere or thedystrophin-associated protein complex. Many muscle proteins, such as α-dystrobrevin, syncoilin, synemin, sarcoglycan, dystroglycan, and sarcospan, colocalize with dystrophin at the costamere. Dystrophin is a protein located between the sarcolemma and the outermost layer of myofilaments in the muscle fiber (myofiber) . It is a cohesive protein, linking actin filaments to another support protein that resides on the inside surface of each muscle fiber’s plasma membrane (sarcolemma) .

Synonyms

Dystrophin; DMD

Gene Name

DMD

Gene ID

1756

UniProt

P11532

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Dystrophin recombinant protein (Position: H3076-D3404) . Human Dystrophin shares 100% amino acid (aa) sequence identity with mouse Dystrophin.

Clonality

Polyclonal

Tissue Specificity

Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Isoform 5 is expressed in heart, brain, liver, testis and hepatoma cells. Most tissues contain transcripts of multiple isoforms, however only isoform 5 is detected in heart and liver. .

Applications

Flow Cytometry, IHC, IHC-F, ICC, WB

Field of Research

Biochemicals, Chemical Type, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin- associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission. .

References & Citations

1. Tennyson CN, Klamut HJ, Worton RG (1995) . The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced. Nature Genetics 9 (2) : 184–90. 2. García-Pelagio KP, Bloch RJ, Ortega A, González-Serratos H (2011) . Biomechanics of the sarcolemma and costameres in single skeletal muscle fibers from normal and dystrophin- null mice. J Muscle Res Cell Motil 31 (5-6) : 323–336. 3. Strachan T and Read AP, 1999. Human molecular genetics, BIOS Scientific, New York, USA

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

426750 MW

Observed Molecular Weight

427 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Dystrophin

Subcellular Location

Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell junction, synapse, postsynaptic cell membrane . In muscle cells, sarcolemma localization requires the presence of ANK2, while localization to costameres requires the presence of ANK3. Localizes to neuromuscular junctions (NMJs) in the presence of ANK2 (By similarity) . .

Sequence Similarities

Contains 2 CH (calponin-homology) domains.

Protein Name

Dystrophin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.