Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband®

About this product

Boster Bio Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband® catalog # PB9277. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9277

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.

Synonyms

Basement membrane-specific heparan sulfate proteoglycan core protein; HSPG; Perlecan; PLC; Endorepellin; LG3 peptide; HSPG2

Gene Name

HSPG2

Gene ID

3339

UniProt

P98160

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human HSPG2 recombinant protein (Position: F524-K701) . Human HSPG2 shares 86% amino acid (aa) sequence identity with mouse HSPG2.

Clonality

Polyclonal

Tissue Specificity

Found in the basement membranes.

Applications

IHC, WB

Field of Research

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.

References & Citations

1. Arikawa-Hirasawa E, Le AH, Nishino I, Nonaka I, Ho NC, Francomano CA et al. (May 2002) . 2. Iozzo RV (1994) . Perlecan: a gem of a proteoglycan. Matrix Biol. 14 (3) : 203–8. 3. Kallunki P, Eddy RL, Byers MG, Kestilä M, Shows TB, Tryggvason K (October 1991) . Cloning of human heparan sulfate proteoglycan core protein, assignment of the gene (HSPG2) to 1p36.1----p35 and identification of a BamHI restriction fragment length polymorphism. Genomics 11 (2) : 389–96.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

468830 MW

Observed Molecular Weight

469 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Basement membrane-specific heparan sulfate proteoglycan core protein

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Sequence Similarities

Contains 4 EGF-like domains.

Protein Name

Basement membrane-specific heparan sulfate proteoglycan core protein

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.