Anti-Profilin 1/PFN1 Antibody Picoband®

About this product

Boster Bio Anti-Profilin 1/PFN1 Antibody Picoband® catalog # PB9313. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9313

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Profilin-1 is a protein that in humans is encoded by the PFN1 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. PFN1 is mapped to 17p13.2. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. This gene is a 140-amino acid protein and major growth regulator of filamentous (F) -actin through its binding of monomeric (G) -actin. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.

Synonyms

Profilin-1; Epididymis tissue protein Li 184a; Profilin I; PFN1

Gene Name

PFN1

Gene ID

5216

UniProt

P07737

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 1, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in epididymis (at protein level) . .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Actin, Actin Assembly, Actin, etc., Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Cell Type Marker, Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Neuron Marker, Neuroscience, Oncoproteins/Suppressors, Signal Transduction, Subcellular Markers, Synapse Marker, Tags & Cell Markers, Tumor Suppressors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR. .

References & Citations

1. Entrez Gene: PFN1 profilin 1 2. Kwiatkowski, D. J., Aklog, L., Ledbetter, D. H., Morton, C. C. Identification of the functional profilin gene, its localization to chromosome subband 17p13.3, and demonstration of its deletion in some patients with Miller-Dieker syndrome. Am. J. Hum. Genet. 46: 559-567, 1990. 3. Mockrin, S. C., Korn, E. D. Acanthamoeba profilin interacts with G-actin to increase the rate of exchange of actin-bound adenosine 5-prime-triphosphate. Biochemistry 19: 5359-5362, 1980.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

15054 MW

Observed Molecular Weight

15 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Profilin-1

Subcellular Location

Cytoplasm, cytoskeleton.

Sequence Similarities

Contains 1 PAC (PAS-associated C-terminal) domain.

Protein Name

Profilin-1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.