Anti-PBK Antibody Picoband®

About this product

Boster Bio Anti-PBK Antibody Picoband® catalog # PB9310. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9310

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lymphokine-activated killer T-cell-originated protein kinase, also known as TOPK or PBK, is an enzyme that in humans is encoded by the PBK gene. The protein encoded by this gene is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. It is mapped to 8p21.1. PBK can mediate cell growth through histone H3 modification. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis.

Synonyms

Lymphokine-activated killer T-cell-originated protein kinase;2.7.12.2; Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase; PBK; TOPK

Gene Name

PBK

Gene ID

55872

UniProt

Q96KB5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PBK recombinant protein (Position: N71-V322) . Human PBK shares 89% amino acid (aa) sequence identity with mouse PBK.

Clonality

Polyclonal

Tissue Specificity

Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cell Division, Cytokinesis, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, MAPK Pathway, p53 Pathway, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. .

References & Citations

1. Abe Y, Matsumoto S, Kito K, Ueda N (Aug 2000) . Cloning and expression of a novel MAPKK-like protein kinase, lymphokine-activated killer T-cell-originated protein kinase, specifically expressed in the testis and activated lymphoid cells. J Biol Chem 275 (28) : 21525–31. 2. Entrez Gene: PBK PDZ binding kinase 3. Park, J.-H., Lin, M.-L., Nishidate, T., Nakamura, Y., Katagiri, T. PDZ-binding kinase/T-LAK cell-originated protein kinase, a putative cancer/testis antigen with an oncogenic activity in breast cancer. Cancer Res. 66: 9186-9195, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36085 MW

Observed Molecular Weight

40 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Lymphokine-activated killer T-cell-originated protein kinase

Subcellular Location

Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.

Sequence Similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Protein Name

Lymphokine-activated killer T-cell-originated protein kinase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.