Anti-PARP/PARP1 Antibody Picoband®

About this product

Boster Bio Anti-PARP/PARP1 Antibody Picoband® catalog # PB9309. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9309

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Poly [ADP-ribose] polymerase 1 (PARP1), also known as ADPRT or PPOL is an enzyme that in humans is encoded by the PARP1 gene. PARP1 gene is mapped to 1q42.12. This gene encodes a chromatin-associated enzyme, poly (ADP-ribosyl) transferase, which modifies various nuclear proteins by poly (ADP-ribosyl) ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Synonyms

Poly [ADP-ribose] polymerase 1; PARP-1;2.4.2.30; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD (+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; PARP1; ADPRT, PPOL

Gene Name

PARP1

Gene ID

142

UniProt

P09874

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PARP recombinant protein (Position: Q670-R858) . Human PARP shares 94% and 95% amino acid (aa) sequence identity with mouse and rat PARP, respectively.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain (at protein level) . Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl) ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly (ADP- ribosyl) ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to ly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. .

References & Citations

1. Ambrose, H. E., Willimott, S., Beswick, R. W., Dantzer, F., Menissier de Murcia, J., Yelamos, J., Wagner, S. D. Poly (ADP-ribose) polymerase-1 (Parp-1) -deficient mice demonstrate abnormal antibody responses. Immunology 127: 178-186, 2008. 2. Doege, C. A., Inoue, K., Yamashita, T., Rhee, D. B., Travis, S., Fujita, R., Guarnieri, P., Bhagat, G., Vanti, W. B., Shih, A., Levine, R. L., Nik, S., Chen, E. I., Abeliovich, A. Early-stage epigenetic modification during somatic cell reprogramming by Parp1 and Tet2. Nature 488: 652-655, 2012.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

113084 MW

Observed Molecular Weight

120 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Poly [ADP-ribose] polymerase 1

Subcellular Location

Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.

Sequence Similarities

Contains 1 BRCT domain.

Protein Name

Poly [ADP-ribose] polymerase 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.