Anti-P2RX4 Antibody Picoband®

About this product

Boster Bio Anti-P2RX4 Antibody Picoband® catalog # PB9304. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9304

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Purinoceptor P2X4, also called P2X4R is a protein that in humans is encoded by the P2RX4 gene. This gene belongs to the family of purinoceptors for ATP. P2RX4 was mapped to 12q24.32 by fluorescence in situ hybridization. P2RX4 is a receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. P2X4 receptor in hyperactive microglia is necessary for tactile allodynia after nerve injury and is sufficient to produce tactile allodynia in normal animals. P2X4 recetors have beeb implicated in the regulation of cardiac function, ATP-mediated cell death, synaptic strengthening, and activating of the inflammasome in response to injury.

Synonyms

P2X purinoceptor 4; P2X4; ATP receptor; Purinergic receptor; P2RX4

Gene Name

P2RX4

Gene ID

5025

UniProt

Q99571

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human P2RX4 recombinant protein (Position: N262-Q388) . Human P2RX4 shares 91% and 90% amino acid (aa) sequence identity with mouse and rat P2RX4, respectively.

Clonality

Polyclonal

Applications

WB

Field of Research

Cardiovascular, Cell Biology, Ligand-Gated Ion Channels, Neuroscience, Neurotransmission, Receptors / Channels

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. .

References & Citations

1. Adrian, K., Bernhard, M. K., Breitinger, H.-G., Ogilvie, A. Expression of purinergic receptors (ionotropic P2X1-7 and metabotropic P2Y1-11) during myeloid differentiation of HL60 cells. Biochim. Biophys. Acta 1492: 127-138, 2000. 2. Kawate, T., Michel, J. C., Birdsong, W. T., Gouaux, E. Crystal structure of the ATP-gated P2X4 ion channel in the closed state. Nature 460: 592-598, 2009. 3. Wilson, S. G., Adam, G., Langdown, M., Reneland, R., Braun, A., Andrew, T., Surdulescu, G. L., Norberg, M., Dudbridge, F., Reed, P. W., Sambrook, P. N., Kleyn, P. W., Spector, T. D. Linkage and potential association of obesity-related phenotypes with two genes on chromosome 12q24 in a female dizygous twin cohort. Europ. J. Hum. Genet. 14: 340-348, 2006. 4. Yamamoto, K., Sokabe, T., Matsumoto, T., Yoshimura, K., Shibata, M., Ohura, N., Fukuda, T., Sato, T., Sekine, K., Kato, S., Isshiki, M., Fujita, T., Kobayashi, M., Kawamura, K., Masuda, H., Kamiya, A., Ando, J. Impaired flow-dependent control of vascular tone and remodeling in P2X4-deficient mice. Nature Med. 12: 133-137, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

43369 MW

Observed Molecular Weight

72 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

P2X purinoceptor 4

Subcellular Location

Membrane ; Multi-pass membrane protein .

Sequence Similarities

Belongs to the P2X receptor family.

Protein Name

P2X purinoceptor 4

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.