Anti-Nkx2.5/NKX2-5 Antibody Picoband®

About this product

Boster Bio Anti-Nkx2.5/NKX2-5 Antibody Picoband® catalog # PB9293. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9293

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Homeobox protein Nkx-2.5, also known as NKX2E or CSX is a protein that in humans is encoded by the NKX2-5 gene. It is mapped to 5q35.1. Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. Nkx2.5 and Tbx5 ly bound to the promoter of the gene encoding cardiac-specific natriuretic peptide precursor type A (NPPA) in tandem, and both transcription factors showed synergistic activation. The cardiac homeobox protein Nkx2.5 is essential in cardiac development, and mutations in CSX (which encodes Nkx2.5) cause various congenital heart malformations.

Synonyms

Homeobox protein Nkx-2.5; Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E; NKX2-5; CSX, NKX2.5, NKX2E

Gene Name

NKX2-5

Gene ID

1482

UniProt

P52952

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Nkx2.5, different from the related mouse and rat sequences by five amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed only in the heart.

Applications

WB

Field of Research

Cardiogenesis, Cardiovascular, Developmental Biology, Lineage Markers, Lineage Specification, Mesenchymal Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Stem Cells, Transcription Factors/Regulators

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity) . It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity) . It is required for spleen development. .

References & Citations

1. Hiroi, Y., Kudoh, S., Monzen, K., Ikeda, Y., Yazaki, Y., Nagai, R., Komuro, I. Tbx5 associates withNkx2-5 and synergistically promotes cardiomyocyte differentiation. Nature Genet. 28: 276-280, 2001. 2. Shiojima, I., Komuro, I., Inazawa, J., Nakahori, Y., Matsushita, I., Abe, T., Nagai, R., Yazaki, Y.Assignment of cardiac homeobox gene CSX to human chromosome 5q34. Genomics 27: 204-206, 1995. 3. Turbay D, Wechsler SB, Blanchard KM, Izumo S (January 1997) . Molecular cloning, chromosomal mapping, and characterization of the human cardiac-specific homeobox gene hCsx. Mol Med 2 (1) : 86–96.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34918 MW

Observed Molecular Weight

35 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Homeobox protein Nkx-2.5

Subcellular Location

Nucleus .

Sequence Similarities

Belongs to the NK-2 homeobox family.

Protein Name

Homeobox protein Nkx-2.5

Isotype

Rabbit IgG

Contents

243