Anti-Galectin 3/LGALS3 Antibody Picoband®

About this product

Boster Bio Anti-Galectin 3/LGALS3 Antibody Picoband® catalog # PB9279. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9279

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, it located on chromosome 14, locus q21– q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

Synonyms

Galectin-3; Gal-3;35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; IgE-binding protein; L-34 galactoside-binding lectin; Laminin-binding protein; Lectin L-29; Mac-2 antigen; Lgals3

Gene Name

LGALS3

Gene ID

16854

UniProt

P16110

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived mouse Galectin 3 recombinant protein (Position: K153-I264) . Mouse Galectin 3 shares 88% and 91% amino acid (aa) sequence identity with human and rat Galectin 3, respectively.

Clonality

Polyclonal

Tissue Specificity

The highest levels are found in activated macrophages.

Applications

IHC, WB

Field of Research

Cancer, Cell Cycle, Cell Division, Oncoproteins, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells (By similarity) . .

References & Citations

1. Dumic J, Dabelic S, Flögel M. (2006) . Galectin-3: an open-ended story.. BBA General Subjects 1760 (4) : 616–635. 2. Liu F, Patterson RJ, Wang JL. (2002) . Intracellular function of galectins.. BBA General Subjects 1572 (2-3) : 263–273. 3. Cooper D. (2002) . Galectinomics: finding themes in complexity.. BBA General Subjects 1572 (2-3) : 209–231. 4. Raimond J, Zimonjic DB, Mignon C, et al. (1997) . Mapping of the galectin-3 gene (LGALS3) to human chromosome 14 at region 14q21—22.. Mamm. Genome 8 (9) : 706–707.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

27515 MW

Observed Molecular Weight

29 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Galectin-3

Subcellular Location

Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface.

Sequence Similarities

Contains 3 cystatin kininogen-type domains.

Protein Name

Galectin-3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.