Anti-IKB beta/NFKBIB Antibody Picoband®

About this product

Boster Bio Anti-IKB beta/NFKBIB Antibody Picoband® catalog # PB9292. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9292

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NF-kappa-B inhibitor beta, also known as IKBB or TRIP9, is a protein that in humans is encoded by the NFKBIB gene. The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. This gene is mapped to 19q13.2. It has been found that in vivo, NFKBIB serves both to inhibit and to facilitate the inflammatory response. NFKBIB degradation releases NF-kappa-B dimers, which upregulate proinflammatory target genes such as TNF-alpha. Surprisingly, absence of NFKBIB results in a dramatic reduction of TNF-alpha in response to lipopolysaccharide, even though activation of NF-kappa-B is normal.

Synonyms

NF-kappa-B inhibitor beta; NF-kappa-BIB; I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9; NFKBIB; IKBB, TRIP9

Gene Name

NFKBIB

Gene ID

4793

UniProt

Q15653

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human IKB beta recombinant protein (Position: E56-E237) . Human IKB beta shares 82% and 80% amino acid (aa) sequence identity with mouse and rat IKB beta, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues examined.

Applications

IHC, WB

Field of Research

Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, NFKB Pathway, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, TLR Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA- dependent inactivation. Association with inhibitor kappa B- interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

References & Citations

1. Okamoto T, Ono T, Hori M, Yang J, Tetsuka T, Kawabe T et al. (November 1998) . Assignment of the IkappaB-beta gene NFKBIB to human chromosome band 19q13.1 by in situ hybridization. Cytogenet Cell Genet 82 (1–2) : 105–6. 2. Rao, P., Hayden, M. S., Long, M., Scott, M. L., West, A. P., Zhang, D., Oeckinghaus, A., Lynch, C., Hoffmann, A., Baltimore, D., Ghosh, S. I-kappa-B-beta acts to inhibit and activate gene expression during the inflammatory response. Nature 466: 1115-1119, 2010.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

38 kDa

Observed Molecular Weight

45-48 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

NF-kappa-B inhibitor beta

Subcellular Location

Cytoplasm . Nucleus .

Sequence Similarities

Belongs to the NF-kappa-B inhibitor family.

Protein Name

NF-kappa-B inhibitor beta

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.