Anti-IKB alpha/NFKBIA Antibody Picoband®

About this product

Boster Bio Anti-IKB alpha/NFKBIA Antibody Picoband® catalog # PB9291. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9291

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NFKBIA, also called IKBA or MAD-3, is one member of a family of cellular proteins that function to inhibit the NF-κ B transcription factor. It is mapped to 14q13.2. NFKBIA inhibits NF-κ B by masking the nuclear localization signals (NLS) of NF-κ B proteins and keeping them sequestered in an inactive state in the cytoplasm. It moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. The effect of the nonpathogenic bacteria is specific to the SCF complex substrates CTNNB1 and NFKBIA. This may help to explain the beneficial effects of treatment of inflammatory bowel disease with nonpathogenic probiotic enteric organisms. In addition, NFKBIA blocks the ability of NF-κ B transcription factors to bind to DNA, which is required for NF-κ B's proper functioning.

Synonyms

NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA, MAD3, NFKBI

Gene Name

NFKBIA

Gene ID

4792

UniProt

P25963

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human IKB alpha recombinant protein (Position: Q3-Q112) . Human IKB alpha shares 87% and 86% amino acid (aa) sequence identity with mouse and rat IKB alpha, respectively.

Clonality

Polyclonal

Applications

WB

Field of Research

Cancer, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, NFKB Pathway, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, TLR Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. .

References & Citations

1. Jacobs MD, Harrison SC (1998) . Structure of an IkappaBalpha/NF-kappaB complex. Cell 95 (6) : 749–58. 2. Neish, A. S.., Gewirtz, A. T., Zeng, H., Young, A. N., Hobert, M. E., Karmali, V., Rao, A. S., Madara, J. L. Prokaryotic regulation of epithelial responses by inhibition of I-kappa-B-alpha ubiquitination. Science 289: 1560-1563, 2000. 3. Verma IM, Stevenson JK, Schwarz EM, Van Antwerp D, Miyamoto S (1995) . Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev.9 (22) : 2723–35.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36 kDa

Observed Molecular Weight

39 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

NF-kappa-B inhibitor alpha

Subcellular Location

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. .

Sequence Similarities

Belongs to the NF-kappa-B inhibitor family.

Protein Name

NF-kappa-B inhibitor alpha

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.