Anti-c-Myb Antibody Picoband®

About this product

Boster Bio Anti-c-Myb Antibody Picoband® catalog # PB9288. Tested in Flow Cytometry, IHC, IF, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9288

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Myb proto-oncogene protein, also known as transcriptional activator Myb, is a protein that in humans is encoded by the MYB gene. It is a member of the MYB (myeloblastosis) family of transcription factors. This gene is mapped to 6q23.3.The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis, including the regulation of miR-155 in B-cells. MYB is also a critical target of MIR150.

Synonyms

Transcriptional activator Myb; Proto-oncogene c-Myb; MYB

Gene Name

MYB

Gene ID

4602

UniProt

P10242

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human c-Myb recombinant protein (Position: M1-E201) . Human c-Myb shares 100% amino acid (aa) sequence identity with mouse c-Myb.

Clonality

Polyclonal

Tissue Specificity

Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level) . .

Applications

Flow Cytometry, IHC, IF, WB

Field of Research

Cancer, Cancer Susceptibility,2339, Epigenetics and Nuclear Signaling, Oncoproteins, Oncoproteins/Suppressors, Proto-Oncogenes, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

References & Citations

1. Chen Y, Xu H, Liu J, Zhang C, Leutz A, Mo X (Jul 2007) . The c-Myb functions as a downstream target of PDGF-mediated survival signal in vascular smooth muscle cells. Biochem Biophys Res Commun 360 (2) : 433–6. 2. Xiao, C., Calado, D. P., Galler, G., Thai, T.-H., Patterson, H. C., Wang, J., Rajewsky, N., Bender, T. P., Rajewsky, K. MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell 131: 146-159, 2007. Xiao, C., Calado, D. P., Galler, G., Thai, T.-H., Patterson, H. C., Wang, J., Rajewsky, N., Bender, T. P., Rajewsky, K. MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell 131: 146-159, 2007. 3. Vargova K, Curik N, Burda P, Basova P, Kulvait V, Pospisil V, Savvulidi F, Kokavec J, Necas E, Berkova A, Obrtlikova P, Karban J, Mraz M, Pospisilova S, Mayer J, Trneny M, Zavadil J, Stopka T (April 2011) . MYB transcriptionally regulates the miR-155 host gene in chronic lymphocytic leukemia. Blood 117 (14) : 3816–25.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

72 kDa

Observed Molecular Weight

70-80 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Transcriptional activator Myb

Subcellular Location

Nucleus .

Sequence Similarities

Contains 3 HTH myb-type DNA-binding domains.

Protein Name

Transcriptional activator Myb

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.