Anti-Mesothelin/MSLN Antibody Picoband®

About this product

Boster Bio Anti-Mesothelin/MSLN Antibody Picoband® catalog # PB9287. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9287

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.

Synonyms

Mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; Megakaryocyte-potentiating factor; MPF; Mesothelin, cleaved form; MSLN; MPF

Gene Name

MSLN

Gene ID

10232

UniProt

Q13421

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Mesothelin recombinant protein (Position: K306-L576) . Human Mesothelin shares 57% and 56% amino acid (aa) sequence identity with mouse and rat Mesothelin, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level) . .

Applications

ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB

Field of Research

Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Membrane-anchored forms may play a role in cellular adhesion.

References & Citations

1. Bera, T. K., Pastan, I. Mesothelin is not required for normal mouse development or reproduction. Molec. Cell. Biol. 20: 2902-2906, 2000. 2. Scholler, N., Fu, N., Yang, Y., Ye, Z., Goodman, G. E., Hellstrom, K. E., Hellstrom, I. Soluble member (s) of the mesothelin/megakaryocyte potentiating factor family are detectable in sera from patients with ovarian carcinoma. Proc. Nat. Acad. Sci. 96: 11531-11536, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68986 MW

Observed Molecular Weight

69 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Mesothelin

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.

Protein Name

Mesothelin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.