Anti-Apolipoprotein E/APOE Antibody Picoband®

About this product

Boster Bio Anti-Apolipoprotein E/APOE Antibody Picoband® catalog # PB9327. Tested in ELISA, Flow Cytometry, IHC, IF, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9327

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.

Synonyms

Apolipoprotein E; Apo-E; APOE

Gene Name

APOE

Gene ID

348

UniProt

P02649

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Apolipoprotein E recombinant protein (Position: K19-H317) . Human Apolipoprotein E shares 73% and 72% amino acid (aa) sequence identity with mouse and rat Apolipoprotein E, respectively.

Clonality

Polyclonal

Tissue Specificity

Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL) . APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

Applications

ELISA (Cap), Flow Cytometry, IF, IHC, WB

Field of Research

Cardiovascular, Lipid Metabolism, Lipids/Lipoproteins, Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.

References & Citations

1. Calabuig-Navarro MV, et al. Apolipoprotein E genotype has a modest impact on the postprandial plasma response to meals of varying fat composition in healthy men in a randomized controlled trial. J Nutr, 2014 Nov. 2. APOE ε variants increase risk of warfarin-related intracerebral hemorrhage.Falcone GJ, et al. Neurology, 2014 Sep 23.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36154 MW

Observed Molecular Weight

36 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Apolipoprotein E

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the apolipoprotein A1/A4/E family.

Protein Name

Apolipoprotein E

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.