Anti-ATP5H Antibody Picoband®

About this product

Boster Bio Anti-ATP5H Antibody Picoband® catalog # PB9328. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9328

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) . This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.

Synonyms

ATP synthase subunit d, mitochondrial; ATPase subunit d; ATP5H; My032

Gene Name

ATP5H

Gene ID

10476

UniProt

O75947

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human ATP5H recombinant protein (Position: A2-L161) . Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H, respectively.

Clonality

Polyclonal

Tissue Specificity

Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL) . APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

Applications

IF, IHC, ICC, WB

Field of Research

Mitochondria, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Organelles, Oxidative Phosphorylation, Pathways and Processes, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Mitochondrial membrane ATP synthase (F (1) F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F (0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha (3) beta (3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

References & Citations

1. Boada M, et al. ATP5H/KCTD2 locus is associated with Alzheimer's disease risk. Mol Psychiatry, 2014 Jun. 2. Kim DW, et al. Identification of mitochondrial F (1) F (0) -ATP synthase interacting with galectin-3 in colon cancer cells. Cancer Sci, 2008 Oct.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

18491 MW

Observed Molecular Weight

22 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

ATP synthase subunit d, mitochondrial

Subcellular Location

Mitochondrion. Mitochondrion inner membrane.

Sequence Similarities

Belongs to the apolipoprotein A1/A4/E family.

Protein Name

ATP synthase subunit d, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.