Anti-Calpain 1/CAPN1 Antibody Picoband®

About this product

Boster Bio Anti-Calpain 1/CAPN1 Antibody Picoband® catalog # PB9330. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9330

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CAPN1 is also known as CANP or muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

Synonyms

Calpain-1 catalytic subunit;3.4.22.52; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; muCANP; CAPN1; CANPL1; PIG30

Gene Name

CAPN1

Gene ID

823

UniProt

P07384

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555) . Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Calcium Signaling, Cell Biology, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

References & Citations

1. Smith IJ, et al. Calpain activity is increased in skeletal muscle from gastric cancer patients with no or minimal weight loss. Muscle Nerve, 2011 Mar. 2. Wang JC, et al. Proteolysis by endogenous calpain I leads to the activation of calcineurin in human heart. Clin Lab, 2012.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

81890 MW

Observed Molecular Weight

82 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Calpain-1 catalytic subunit

Subcellular Location

Cytoplasm . Cell membrane . Translocates to the plasma membrane upon Ca (2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719) . .

Sequence Similarities

Belongs to the peptidase C2 family.

Protein Name

Calpain-1 catalytic subunit

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.