Anti-ERAB/HSD17B10 Antibody Picoband®

About this product

Boster Bio Anti-ERAB/HSD17B10 Antibody Picoband® catalog # PB9336. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9336

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ERAB is also known as HSD17B10 or HADH2. This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Synonyms

3-hydroxyacyl-CoA dehydrogenase type-2;1.1.1.35;17-beta-hydroxysteroid dehydrogenase 10;17-beta-HSD 10;1.1.1.51;3-hydroxy-2-methylbutyryl-CoA dehydrogenase;1.1.1.178;3-hydroxyacyl-CoA dehydrogenase type II; Endoplasmic reticulum-associated amyloid beta-peptide-binding protein; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Short chain dehydrogenase/reductase family 5C member 1; Short-chain type dehydrogenase/reductase XH98G2; Type II HADH; HSD17B10; ERAB, HADH2, MRPP2, SCHAD, SDR5C1, XH98G2

Gene Name

HSD17B10

Gene ID

3028

UniProt

Q99714

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human ERAB recombinant protein (Position: E48-P261) . Human ERAB shares 87% and 88% amino acid (aa) sequence identity with mouse and rat ERAB, respectively.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed in normal tissues but is overexpressed in neurons affected in AD. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Ligand-Gated Ion Channels, Neuroscience, Neurotransmission, Receptors / Channels

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. Catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD) . .

References & Citations

1. Deutschmann AJ, et al. Mutation or knock-down of 17β-hydroxysteroid dehydrogenase type 10 cause loss of MRPP1 and impaired processing of mitochondrial heavy strand transcripts. Hum Mol Genet, 2014 Jul 1. 2. Yang SY, et al. Roles of 17β-hydroxysteroid dehydrogenase type 10 in neurodegenerative disorders. J Steroid Biochem Mol Biol, 2014 Sep.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26923 MW

Observed Molecular Weight

25 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

3-hydroxyacyl-CoA dehydrogenase type-2

Subcellular Location

Mitochondrion .

Protein Name

3-hydroxyacyl-CoA dehydrogenase type-2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.