Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband®

About this product

Boster Bio Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband® catalog # PB9342. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9342

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31) . GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus) .

Synonyms

Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1; NR3C1; GRL

Gene Name

NR3C1

Gene ID

2908

UniProt

P04150

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human NR3C1 recombinant protein (Position: A20-F199) . Human NR3C1 shares 80% and 74% amino acid (aa) sequence identity with mouse and rat NR3C1, respectively.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. .

Applications

IHC, WB

Field of Research

2339, Domain Families, Epigenetics and Nuclear Signaling, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription, Zinc Finger

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for glucocorticoids (GC) . Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression. .

References & Citations

1. Lu NZ, Wardell SE, Burnstein KL, Defranco D, Fuller PJ, Giguere V, Hochberg RB, McKay L, Renoir JM, Weigel NL, Wilson EM, McDonnell DP, Cidlowski JA (2006) . International Union of Pharmacology. LXV. The pharmacology and classification of the nuclear receptor superfamily: glucocorticoid, mineralocorticoid, progesterone, and androgen receptors. Pharmacol Revl 58 (4) : 782–97. 2. Rhen T, Cidlowski JA (October 2005) . Antiinflammatory action of glucocorticoids--new mechanisms for old drugs. N. Engl. J. Med. 353 (16) : 1711–23. 3. Hollenberg SM, Weinberger C, Ong ES, Cerelli G, Oro A, Lebo R, Thompson EB, Rosenfeld MG, Evans RM (1985) . Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature 318 (6047) : 635–41.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

85659 MW

Observed Molecular Weight

100 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Glucocorticoid receptor

Subcellular Location

Cytoplasm . Mitochondrion. Nucleus . Cytoplasmic in the absence of ligand, nuclear after ligand- binding.

Sequence Similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily.

Protein Name

Glucocorticoid receptor

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.