Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband®

About this product

Boster Bio Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband® catalog # PB9345. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9345

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PLAT is also known as tPA. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Synonyms

Tissue-type plasminogen activator; t-PA; t-plasminogen activator; tPA;3.4.21.68; Alteplase; Reteplase; Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B; PLAT

Gene Name

PLAT

Gene ID

5327

UniProt

P00750

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human TPA recombinant protein (Position: H366-P562) . Human TPA shares 83% and 84% amino acid (aa) sequence identity with mouse and rat TPA, respectively.

Clonality

Polyclonal

Tissue Specificity

Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

Applications

WB

Field of Research

2339, Developmental Biology, Embryogenesis, Epigenetics and Nuclear Signaling, Lineage Markers, Lineage Specification, Nervous System Development, Neurogenesis, Neurology Process, Neuroscience, Organogenesis, Sensory System, Stem Cells, Transcription, Visual System

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a role in facilitating neuronal migration.

References & Citations

1. Marsh EB, et al. Serum creatinine may indicate risk of symptomatic intracranial hemorrhage after intravenous tissue plasminogen activator (IV tPA) . Medicine (Baltimore), 2013 Nov. 2. Valle-Garay E, et al. tPA Alu (I/D) polymorphism associates with bacterial osteomyelitis. J Infect Dis, 2013 Jul 15.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

62917 MW

Observed Molecular Weight

63-69 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Tissue-type plasminogen activator

Subcellular Location

Secreted, extracellular space.

Sequence Similarities

Belongs to the peptidase S1 family.

Protein Name

Tissue-type plasminogen activator

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.