Anti-PP2A-alpha/PPP2CA Antibody Picoband®

About this product

Boster Bio Anti-PP2A-alpha/PPP2CA Antibody Picoband® catalog # PB9347. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9347

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.

Synonyms

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha;3.1.3.16; Replication protein C; RP-C; PPP2CA

Gene Name

PPP2CA

Gene ID

5515

UniProt

P67775

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

PP2A-alpha shares 100% cross-reactivity with PPP2CB.

Immunogen

E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309) . Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha.

Clonality

Polyclonal

Tissue Specificity

Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

Applications

IF, IHC, ICC, WB, Flow Cytometry

Field of Research

Epigenetics and Nuclear Signaling, Intracellular Signaling, Kinases/Phosphatases, Neuroscience, Neurotransmission, Protein Phosphorylation, Ser/Thr Phosphatases, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

PP2A is the major phosphatase for microtubule-associated proteins (MAPs) . PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity) . Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. .

References & Citations

1. Stone SR, Mayer R, Wernet W, Maurer F, Hofsteenge J, Hemmings BA. The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A alpha catalytic subunit. Nucleic Acids Res. 1988 Dec 9; 16 (23) :11365. 2. Jones TA, Barker HM, da Cruz e Silva EF, Mayer-Jaekel RE, Hemmings BA, Spurr NK, Sheer D, Cohen PT. Localization of the genes encoding the catalytic subunits of protein phosphatase 2A to human chromosome bands 5q23-->q31 and 8p12-->p11.2, respectively. Cytogenet Cell Genet. 1993; 63 (1) :35-41. 3. Khew-Goodall Y, Mayer RE, Maurer F, Stone SR, Hemmings BA. Structure and transcriptional regulation of protein phosphatase 2A catalytic subunit genes. Biochemistry. 1991 Jan 8; 30 (1) :89-97. 4. Arino J, Woon CW, Brautigan DL, Miller TB Jr, Johnson GL. Human liver phosphatase 2A: cDNA and amino acid sequence of two catalytic subunit isotypes. Proc Natl Acad Sci U S A. 1988 Jun; 85 (12) :4252-6. 5. Bryant JC, Westphal RS, Wadzinski BE. Methylated C-terminal leucine residue of PP2A catalytic subunit is important for binding of regulatory Balpha subunit. Biochem J. 1999 Apr 15; 339 (Pt 2) :241-6. 6. Gergs U, Boknik P, Buchwalow I, Fabritz L, Matus M, Justus I, Hanske G, Schmitz W, Neumann J. Overexpression of the catalytic subunit of protein phosphatase 2A impairs cardiac function. J Biol Chem. 2004 Sep 24; 279 (39) :40827-34.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

35594 MW

Observed Molecular Weight

34 kDa, 36 kDa

Specificity

PP2A-alpha shares 100% cross reactivity with PPP2CB.

Applications Notes

6

Gene Name Synonym

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Subcellular Location

Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity) . .

Sequence Similarities

Belongs to the peptidase S1 family.

Protein Name

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.