Anti-PSCA Antibody Picoband®

About this product

Boster Bio Anti-PSCA Antibody Picoband® catalog # PB9351. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9351

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PSCA is also known as PRO232. This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants.

Synonyms

Prostate stem cell antigen; PSCA; UNQ206/PRO232

Gene Name

PSCA

Gene ID

8000

UniProt

O43653

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PSCA recombinant protein (Position: L21-S95) . Human PSCA shares 66% amino acid (aa) sequence identity with mouse PSCA.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in prostate (basal, secretory and neuroendocrine epithelium cells) . Also found in bladder (transitional epithelium), placenta (trophoblasts), stomach (neuroendocrine cells), colon (neuroendocrine cells) and kidney (collecting ducts) . Overexpressed in prostate cancers and expression is correlated with tumor stage, grade and androgen- independence. Highly expressed in prostate cancer bone metastases. Expressed in gastric epithelial cells, mainly in the isthmus (at protein level) . Not detected in normal intestinal epithelium (at protein level) . .

Applications

WB

Field of Research

Cell Type Markers, Intracellular, Stem Cells, Tags & Cell Markers, Tumor Associated

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro. .

References & Citations

1. Hillerdal V, et al. Systemic treatment with CAR-engineered T cells against PSCA delays subcutaneous tumor growth and prolongs survival of mice. BMC Cancer, 2014 Jan 18. 2. Taeb J, et al. Expression of prostate stem cell antigen (PSCA) in prostate cancer: a tissue microarray study of Iranian patients. Pathol Res Pract, 2014 Jan.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

12912 MW

Observed Molecular Weight

25 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Prostate stem cell antigen

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Sequence Similarities

Contains 1 UPAR/Ly6 domain.

Protein Name

Prostate stem cell antigen

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.