Anti-EAAT2/GLT-1/SLC1A2 Antibody Picoband®

About this product

Boster Bio Anti-EAAT2/GLT-1/SLC1A2 Antibody catalog # RP1065. Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1065

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SLC1A2 is also known as EAAT2 or GLT-1. This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified.

Synonyms

Excitatory amino acid transporter 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2; SLC1A2; EAAT2, GLT1

Gene Name

SLC1A2

Gene ID

6506

UniProt

P43004

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human EAAT2 recombinant protein (Position: T461-K574) . Human EAAT2 shares 96% amino acid (aa) sequence identity with both mouse and rat EAAT2.

Clonality

Polyclonal

Applications

IF, IHC, WB

Field of Research

Neuroscience, Neurotransmitter, Signal Transduction, Transporters

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

References & Citations

1. Jiménez-Jiménez FJ, et al. The solute carrier family 1 (glial high affinity glutamate transporter), member 2 gene, SLC1A2, rs3794087 variant and assessment risk for restless legs syndrome. Sleep Med, 2014 Feb. 2. Poletti S, et al. Effect of early stress on hippocampal gray matter is influenced by a functional polymorphism in EAAT2 in bipolar disorder. Prog Neuropsychopharmacol Biol Psychiatry, 2014 Jun 3. 3. Tanaka K. Role of glutamate transporters in the pathophysiology of major mental illnesses. Nihon Yakurigaku Zasshi, 2013 Dec.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

62104 MW

Observed Molecular Weight

65 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Excitatory amino acid transporter 2

Subcellular Location

Membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the peptidase S1 family. Plasminogen subfamily.

Protein Name

Excitatory amino acid transporter 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.