Anti-NMDAR2B/GRIN2B Antibody Picoband®

About this product

Boster Bio Anti-NMDAR2B/GRIN2B Antibody Picoband® catalog # PB9373. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9373

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The N-methyl-D-aspartate receptor 2B (NMDAR2B), also names as GRIN2B. The sequence of the predicted 1,484-amino acid human protein is 98% and 96% identical to the sequences of the rat and mouse Nmdar2b proteins, respectively. Nmdar2B gene is located on mouse chromosome 6 between Rho and Ly49 centromerically and Glb telomerically. Mapping of the human NMDAR2B receptor subunit gene (GRIN2B) to chromosome 12p12 overexpression of NMDA receptor 2B (NR2B) in the forebrains of transgenic mice leads to enhanced activation of NMDA receptors, facilitating synaptic potentiation in response to stimulation at 10-100 Hz.

Synonyms

Glutamate receptor ionotropic, NMDA 2B; GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B; N-methyl-D-aspartate receptor subunit 3; NR3; hNR3; GRIN2B; NMDAR2B

Gene Name

GRIN2B

Gene ID

2904

UniProt

Q13224

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NMDAR2B recombinant protein (Position: N1076-D1332) . Human NMDAR2B shares 97.7% and 97.3% amino acid (aa) sequence identity with mouse and rat NMDAR2B, respectively.

Clonality

Polyclonal

Tissue Specificity

Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. .

Applications

WB

Field of Research

Receptors / Channels, Sensory System

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity) . .

References & Citations

1. Mandich, P.; Schito, A. M.; Bellone, E.; Antonacci, R.; Finelli, P.; Rocchi, M.; Ajmar, F. : Mapping of the human NMDAR2B receptor subunit gene (GRIN2B) to chromosome 12p12. Genomics 22: 216-218, 1994. 2. Tang, Y.-P.; Shimizu, E.; Dube, G. R.; Rampon, C.; Kerchner, G. A.; Zhuo, M.; Liu, G.; Tsien, J. Z. : Genetic enhancement of learning and memory in mice. Nature 401: 63-69, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

166367 MW

Observed Molecular Weight

180-190 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Glutamate receptor ionotropic, NMDA 2B

Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the secreted frizzled-related protein (sFRP) family.

Protein Name

Glutamate receptor ionotropic, NMDA 2B

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.