Anti-HLA-C Antibody Picoband®

About this product

Boster Bio Anti-HLA-C Antibody Picoband® catalog # PB9377. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9377

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described.

Synonyms

HLA class I histocompatibility antigen, A-2 alpha chain; MHC class I antigen A*2; HLA-A; HLAA

Gene Name

HLA-C

Gene ID

3107

UniProt

Q5QT33

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HLA-C.

Clonality

Polyclonal

Tissue Specificity

Mainly expressed in brain with predominant expression is in the cerebellum, also present in the hippocampus, amygdala, caudate nucleus, corpus callosum, subthalamic nuclei and thalamus. Detected in the heart, skeletal muscle and pancreas.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Adaptive Immunity, Class I, Host-Virus Interaction, Immunology, Interspecies Interaction, Microbiology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the presentation of foreign antigens to the immune system.

References & Citations

1. Ríos A, Rodríguez JM, Moya MR, Galindo PJ, Canteras M, Alvarez MR, Parrilla P (2006) . Associations of HLA-C alleles with multinodular goiters: study in a population from southeastern Spain.. Arch Surg 141 (2) : 123–8. 2. Valés-Gómez M, Reyburn HT, Mandelboim M, Strominger JL (September 1998) . Kinetics of interaction of HLA-C ligands with natural killer cell inhibitory receptors. Immunity 9 (3) : 337–44.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

40922 MW

Observed Molecular Weight

41 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Major histocompatibility complex, class I, C

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the MHC class I family.

Protein Name

MHC class I antigen

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.