Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband®

About this product

Boster Bio Anti-Peroxiredoxin 5/PRDX5 Antibody Picoband® catalog # PB9384. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9384

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PRDX5 (peroxiredoxin 5) also known as AOEB166, ACR1, B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. The PRDX5 gene is mapped to11q13.1. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that PRDX5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. PRDX5 mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. PRDX5 plays a protective role against oxidative stress in human cartilage.

Synonyms

Peroxiredoxin-5, mitochondrial;1.11.1.15; Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20; Thioredoxin reductase; PRDX5; ACR1; SBBI10

Gene Name

PRDX5

Gene ID

25824

UniProt

P30044

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Peroxiredoxin 5 recombinant protein (Position: E66-D198) . Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 5.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Apoptosis, Associated Proteins, Cell Biology, Cell Cycle, Cell Differentiation, Intracellular

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.

References & Citations

1. Budanov, A. V., Sablina, A. A., Feinstein, E., Koonin, E. V., Chumakov, P. M. Regeneration of peroxiredoxins by p53-regulated sestrins, homologs of bacterial AhpD. Science 304: 596-600, 2004. 2. Knoops, B., Clippe, A., Bogard, C., Arsalane, K., Wattiez, R., Hermans, C., Duconseille, E., Falmagne, P., Bernard, A. Cloning and characterization of AOEB166, a novel mammalian antioxidant enzyme of the peroxiredoxin family. J. Biol. Chem. 274: 30451-30458, 1999. 3. Wang, M.-X., Wei, A., Yuan, J., Trickett, A., Knoops, B., Murrell, G. A. C. Expression and regulation of peroxiredoxin 5 in human osteoarthritis. FEBS Lett. 531: 359-362, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

22086 MW

Observed Molecular Weight

22 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Peroxiredoxin-5, mitochondrial

Subcellular Location

Isoform Mitochondrial: Mitochondrion.

Sequence Similarities

Belongs to the peroxiredoxin 2 family.

Protein Name

Peroxiredoxin-5, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.