Anti-ROCK2 Antibody Picoband®

About this product

Boster Bio Anti-ROCK2 Antibody Picoband® catalog # PB9387. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9387

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al. concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.

Synonyms

Rho-associated protein kinase 2;2.7.11.1; Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2; ROCK2; KIAA0619

Gene Name

ROCK2

Gene ID

9475

UniProt

O75116

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ROCK2 recombinant protein (Position: E652-D923) . Human ROCK2 shares 94.9% and 95.2% amino acid (aa) sequence identity with mouse and rat ROCK2, respectively.

Clonality

Polyclonal

Tissue Specificity

Placenta and lung.

Applications

IHC, WB

Field of Research

Apoptosis, Cancer, Cell Biology, Intracellular, Kinases, Protein Phosphorylation, Ser/Thr Kinases, Serine/Threonine Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca (2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation. .

References & Citations

1. Takahashi, N.; Tuiki, H.; Saya, H.; Kaibuchi, K. : Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24. Genomics 55: 235-237, 1999. 2. Thumkeo, D.; Keel, J.; Ishizaki, T.; Hirose, M.; Nonomura, K.; Oshima, H.; Oshima, M.; Taketo, M. M.; Narumiya, S. : Targeted disruption of the mouse Rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death. Molec. Cell. Biol. 23: 5043-5055, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

160900 MW

Observed Molecular Weight

161 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Rho-associated protein kinase 2

Subcellular Location

Cytoplasm. Cell membrane ; Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. .

Sequence Similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein Name

Rho-associated protein kinase 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.