Anti-SFTPA1/2 Antibody Picoband®

About this product

Boster Bio Anti-SFTPA1/2 Antibody Picoband® catalog # PB9390. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9390

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SFTPA1/2 is also known as SP-A. SFTPA1 encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants. SFTPA2 is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10. Mutations in this gene and a highly similar gene located nearby, which affect the highly conserved carbohydrate recognition domain, are associated with idiopathic pulmonary fibrosis. The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication.

Synonyms

Pulmonary surfactant-associated protein A2; PSP-A; PSPA; SP-A; SP-A2;35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; Collectin-5; SFTPA2; COLEC5, PSAP, SFTP1, SFTPA, SFTPA2B

Gene Name

SFTPA2

Gene ID

653509/729238

UniProt

Q8IWL1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPA1/2, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in B-cell lines and T-lymphocytes. .

Applications

IF, IHC, IHC-F, WB

Field of Research

Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.

References & Citations

1. Perez-Gil J, Weaver TE (June 2010) . Pulmonary surfactant pathophysiology: current models and open questions.Physiology (Bethesda) 25 (3) : 132–41. 2. Phelps DS (2001) . Surfactant regulation of host defense function in the lung: a question of balance. Pediatr Pathol Mol Med 20 (4) : 269–92.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26182 MW

Observed Molecular Weight

26 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Pulmonary surfactant-associated protein A2

Subcellular Location

Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.

Sequence Similarities

Belongs to the SFTPA family.

Protein Name

Pulmonary surfactant-associated protein A1/Pulmonary surfactant-associated protein A2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.