Anti-SFRP4 Antibody Picoband®

About this product

Boster Bio Anti-SFRP4 Antibody catalog # RP1067. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1067

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. It is mappaead to 7p14.1. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. And SFRP4 is a hub gene in a Type 2 Diabetes-associated gene coexpression module in human islets, and reduces glucose-induced insulin secretion through decreased β-cell exocytosis.

Synonyms

Secreted frizzled-related protein 4; sFRP-4; Frizzled protein, human endometrium; FrpHE; SFRP4; FRPHE

Gene Name

SFRP4

Gene ID

6424

UniProt

Q6FHJ7

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SFRP4 recombinant protein (Position: E140-V346) . Human SFRP4 shares 90% and 90.3% amino acid (aa) sequence identity with mouse and rat SFRP4, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level) . .

Applications

WB

Field of Research

Secreted, Signaling Pathways, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity) . Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. .

References & Citations

1. Entrez Gene: SFRP4 secreted frizzled-related protein 4. 2. Horvath LG, Lelliott JE, Kench JG et al. (2007) . Secreted frizzled-related protein 4 inhibits proliferation and metastatic potential in prostate cancer.. Prostate 67 (10) : 1081–90.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39827 MW

Observed Molecular Weight

40 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Secreted frizzled-related protein 4

Subcellular Location

Secreted . Cytoplasmic in ovarian tumor cells.

Sequence Similarities

Belongs to the secreted frizzled-related protein (sFRP) family.

Protein Name

Secreted frizzled-related protein 4

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.