Anti-Acetylcholinesterase/ACHE Antibody Picoband®

About this product

Boster Bio Anti-Acetylcholinesterase/ACHE Antibody Picoband® catalog # PB9417. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9417

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ACHE is also known as Acetylcholinesterase. And Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Synonyms

Acetylcholinesterase; AChE;3.1.1.7; ACHE

Gene Name

ACHE

Gene ID

43

UniProt

P22303

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ACHE, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Isoform H is highly expressed in erythrocytes. .

Applications

WB

Field of Research

Neuroscience, Neurotransmitter

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. .

References & Citations

1. Ordentlich A, Barak D, Kronman C, Flashner Y, Leitner M, Segall Y et al. (August 1993) . Dissection of the human acetylcholinesterase active center determinants of substrate specificity. Identification of residues constituting the anionic site, the hydrophobic site, and the acyl pocket. J. Biol. Chem. 268 (23) : 17083–95. 2. Quinn DM (1987) . Acetylcholinesterase: enzyme structure, reaction dynamics, and virtual transition states. Chemical Review87 (5) : 955–79. 3. Sussman JL, Harel M, Silman I (June 1993) . Three-dimensional structure of acetylcholinesterase and of its complexes with anticholinesterase drugs. Chem. Biol. Interact. 87 (1–3) : 187–97.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

67796 MW

Observed Molecular Weight

68 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Acetylcholinesterase

Subcellular Location

Cell junction, synapse . Secreted . Cell membrane ; Peripheral membrane protein .

Sequence Similarities

Belongs to the type-B carboxylesterase/lipase family.

Protein Name

Acetylcholinesterase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.