Anti-APH1a Antibody Picoband®

About this product

Boster Bio Anti-APH1a Antibody Picoband® catalog # PB9421. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9421

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

APH1a encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants.

Synonyms

Gamma-secretase subunit APH-1A; APH-1a; Aph-1alpha; Presenilin-stabilization factor; APH1A; PSF; CGI-78, UNQ579/PRO1141

Gene Name

APH1A

Gene ID

51107

UniProt

Q96BI3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human APH1a, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level. .

Applications

WB

Field of Research

Alzheimer's Disease, Cytoplasmic, Neurodegenerative Disease, Neurology Process, Neuroscience, Signaling Pathways, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein) . It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. .

References & Citations

1. Qin W; Jia L; Zhou A; Zuo X; Cheng Z; Wang F; Shi F; Jia J: The -980C/G polymorphism in APH-1A promoter confers risk of Alzheimer's disease. Aging Cell, 2011 Aug.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

28996 MW

Observed Molecular Weight

29 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Gamma-secretase subunit APH-1A

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi- pass membrane protein. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.

Sequence Similarities

Belongs to the APH-1 family.

Protein Name

Gamma-secretase subunit APH-1A

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.