Anti-Plectin Antibody Picoband®

About this product

Boster Bio Anti-Plectin Antibody Picoband® catalog # PB9430. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9430

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Plectin, known as PLEC, is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes. Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail, revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice ly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD) . Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8) . Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat; exons 1i, 1j and 1h have not been confirmed in human. Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse, rat, and human. The short alternative amino-terminal sequences encoded by the different first exons the targeting of the various isoforms to distinct subcellular locations. As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development, it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, as if custom-made for specific requirements of the particular cells. Concordantly, individual isoforms were found to carry out distinct and specific functions.

Synonyms

Plectin; PCN; PLTN; Hemidesmosomal protein 1; HD1; Plectin-1; PLEC; PLEC1

Gene Name

PLEC

Gene ID

5339

UniProt

Q15149

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Plectin, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed with highest levels in muscle, heart, placenta and spinal cord.

Applications

IF, IHC, ICC, WB

Field of Research

Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity. .

References & Citations

1. Svitkina TM, Verkhovsky AB, Borisy GG (Nov 1996) . Plectin sidearms mediate interaction of intermediate filaments with microtubules and other components of the cytoskeleton. The Journal of Cell Biology135 (4) : 991–1007. 2. Wiche G (Sep 1998) . Role of plectin in cytoskeleton organization and dynamics (ABSTRACT) . Journal of Cell Science. 111 (Pt 17) (17) : 2477–86.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

531791 MW

Observed Molecular Weight

532 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Plectin

Subcellular Location

Cytoplasm, cytoskeleton . Cell junction, hemidesmosome .

Sequence Similarities

Belongs to the plakin or cytolinker family.

Protein Name

Plectin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.