Anti-SDHA Antibody Picoband®

About this product

Boster Bio Anti-SDHA Antibody Picoband® catalog # PB9433. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9433

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Complex II of the mitochondrial respiratory chain, also known as succinate dehydrogenase or succinate:ubiquinone oxidoreductase, consists of 4 nuclear-encoded polypeptides, these are the flavoprotein subunit (SDHA), the iron sulfur protein subunit (SDHB), and the integral membrane protein subunits SDHC and SDHD. SDHA is an acronym for succinate dehydrogenase complex subunit A. The succinate dehydrogenase (SDH) protein complex catalyzes the oxidation of succinate (succinate + ubiquinone => fumarate + ubiquinol) . The SDHA subunit is connected to the SDHB subunit on the hydrophilic, catalytic end of the complex, and weighs 72.7 kDA. Mutations in the SDHA subunit have a distinct pathology from mutations in the SDHB/SDHC/SDHD subunits; it is the only subunit to never have shown tumor suppressor behaviour. Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits. This appears to be due to the expression of two similar SDHA genes (Types I and II) in the paraganglia system.

Synonyms

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial;1.3.5.1; Flavoprotein subunit of complex II; Fp; SDHA; SDH2, SDHF

Gene Name

SDHA

Gene ID

6389

UniProt

P31040

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SDHA recombinant protein (Position: S44-L380) . Human SDHA shares 98.2% and 97.6% amino acid (aa) sequence identity with mouse and rat SDHA, respectively.

Clonality

Polyclonal

Tissue Specificity

Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

Applications

Flow Cytometry, IP, IF, IHC, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Cell Biology, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Mitochondria, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Organelles, Oxidative Phosphorylation, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) . Can act as a tumor suppressor. .

References & Citations

1. Briere, JJ et al. 2005. Human Molecular Genetics. Vol. 14 pg. 3263-3269.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

72692 MW

Observed Molecular Weight

73 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Sequence Similarities

Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Protein Name

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.