Anti-SLC4A1/CD233/Band 3 Antibody Picoband®

About this product

Boster Bio Anti-SLC4A1 Antibody Picoband® catalog # PB9437. Tested in Flow Cytometry, IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9437

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Band 3 is also known as SLC4A1. The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system.

Synonyms

Band 3 anion transport protein; Anion exchange protein 1; AE 1; Anion exchanger 1; Solute carrier family 4 member 1; CD233; SLC4A1; AE1, DI, EPB3

Gene Name

SLC4A1

Gene ID

6521

UniProt

P02730

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human SLC4A1 (Position: E28-N365) . Human SLC4A1 shares 75.7% and 74.5% amino acid (aa) sequence identity with mouse and rat SLC4A1, respectively.

Clonality

Polyclonal

Tissue Specificity

Detected in erythrocytes (at protein level) . Erythrocytes. .

Applications

Flow Cytometry, IF, IHC, IHC-F, WB

Field of Research

Cancer, Cancer Metabolism, Carbohydrate Metabolism, Developmental Biology, Energy Metabolism, Energy Transfer Pathways, Lineage Markers, Lineage Specification, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine. .

References & Citations

1. Jarolim P, Palek J, Amato D, Hassan K, Sapak P, Nurse GT et al. (1991) . Deletion in erythrocyte band 3 gene in malaria-resistant Southeast Asian ovalocytosis.Proc. Natl. Acad. Sci. U.S.A. 88 (24) : 11022–6. 2. Schlüter K, Drenckhahn D (August 1986) .Co-clustering of denatured hemoglobin with band 3: its role in binding of autoantibodies against band 3 to abnormal and aged erythrocytes. Proc. Natl. Acad. Sci. U.S.A. 83 (16) : 6137–41.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

101792 MW

Observed Molecular Weight

102 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Band 3 anion transport protein

Subcellular Location

Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.

Sequence Similarities

Belongs to the anion exchanger (TC 2.A.31) family.

Protein Name

Band 3 anion transport protein

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.