Anti-SOD2 Antibody Picoband®

About this product

Boster Bio Anti-SOD2 Antibody Picoband® catalog # PB9442. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9442

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SOD2 (Superoxide Dismutase 2), also called IPO-B or MNSOD, is a mitochondrial matrix enzyme that scavenges oxygen radicals produced by the extensive oxidation-reduction and electron transport reactions occurring in mitochondria. This gene is a member of the iron/manganese superoxide dismutase family. Using a somatic cell hybrid panel containing different segments of chromosome 6, they demonstrated that SOD2 is located in the region 6q25.3-qter which, together with the FISH analysis, indicated that SOD2 is in the distal portion of 6q25. The SOD2 gene encodes an intramitochondrial free radical scavenging enzyme that is the first line of defense against superoxide produced as a byproduct of oxidative phosphorylation. Adeno-associated viral delivery of the human SOD2 gene resulted in suppression of optic nerve degeneration and rescue of retinal ganglion cells. The findings suggested that reactive oxygen species contributed to retinal cell death and optic nerve damage in mice with complex I deficiency, and that expression of SOD2 attenuated the disease process.

Synonyms

Superoxide dismutase [Mn], mitochondrial;1.15.1.1; SOD2

Gene Name

SOD2

Gene ID

6648

UniProt

P04179

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SOD2, different from the related mouse sequence by one amino acid, and from the related rat sequence by four amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in brain and lymphoblasts. .

Applications

IHC, ICC, WB

Field of Research

Apoptosis, Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Cell Death, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways and Processes, Redox Metabolism, Response To Hypoxia, Signal Transduction, Vasculature

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. .

References & Citations

1. Bastaki, M., Huen, K., Manzanillo, P., Chande, N., Chen, C., Balmes, J. R., Tager, I. B., Holland, N. Genotype-activity relationship for Mn-superoxide dismutase, glutathione peroxidase 1 and catalase in humans. Pharmacogenet. Genomics 16: 279-286, 2006. 2. Creagan, R., Tischfield, J., Ricciuti, F., Ruddle, F. H. Chromosome assignments of genes in man using mouse-human somatic cell hybrids: mitochondrial superoxide dismutase (indophenol oxidase-B, tetrameric) to chromosome 6. Humangenetik 20: 203-209, 1973. 3. Hiroi, S., Harada, H., Nishi, H., Satoh, M., Nagai, R., Kimura, A. Polymorphisms in the SOD2 and HLA-DRB1 genes are associated with nonfamilial idiopathic dilated cardiomyopathy in Japanese. Biochem. Biophys. Res. Commun. 261: 332-339, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

24722 MW

Observed Molecular Weight

24 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Superoxide dismutase [Mn], mitochondrial

Subcellular Location

Mitochondrion matrix.

Sequence Similarities

Belongs to the iron/manganese superoxide dismutase family.

Protein Name

Superoxide dismutase [Mn], mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.