- Catalog number
- PB9465
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- YBX1 (Y box binding protein 1), commonly referred to as YB-1 by researchers, is a human protein. YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414 and contains 18% basic residues and putative nuclear localization signals. The YBX1 gene is mapped on 1p34.2. Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation.
- Synonyms
- Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-1; YBX1; NSEP1, YB1
- Gene Name
- YBX1
- Gene ID
- 4904
- UniProt
- P67809
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human YB1, identical to the related mouse and rat sequences.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in plasma cells in rheumatoid synovium (PubMed:11460154) . Over-expressed in primary breast cancer and metastatic breast cancer cells (PubMed:25280941) . Isoform 1 and isoform 2 are expressed at higher level in proliferating as compared to confluent quiescent endothelial cells (PubMed:19416856) . .
- Applications
- Flow Cytometry, IF, IHC, IHC-F, ICC, WB
- Field of Research
- Cell Type Markers, Chromatin Binding Proteins, Developmental Biology, DNA/RNA Binding, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Intracellular, Stem Cells, Surface Molecules, Transcription, Tumor Associated
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity) . Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro) . May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro. .
- References & Citations
- 1. Bhullar, J., Sollars, V. E. YBX1 expression and function in early hematopoiesis and leukemic cells. Immunogenetics 63: 337-350, 2011. 2. Fukada, T., Tonks, N. K. Identification of YB-1 as a regulator of PTP1B expression: implications for regulation of insulin and cytokine signaling. EMBO J. 22: 479-493, 2003. 3. Makino, Y., Ohga, T., Toh, S., Koike, K., Okumura, K., Wada, M., Kuwano, M., Kohno, K. Structural and functional analysis of the human Y-box binding protein (YB-1) gene promoter. Nucleic Acid Res. 24: 1873-1878, 1996.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 35924 MW
- Observed Molecular Weight
- 50 kDa
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- 6
- Gene Name Synonym
- Nuclease-sensitive element-binding protein 1
- Subcellular Location
- Cytoplasm. Nucleus. Cytoplasmic granule . Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
- Sequence Similarities
- Contains 1 CSD (cold-shock) domain.
- Protein Name
- Nuclease-sensitive element-binding protein 1
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
