Anti-Yes1 Antibody Picoband®

About this product

Boster Bio Anti-Yes1 Antibody Picoband® catalog # PB9466. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9466

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proto-oncogene tyrosine-protein kinase Yes is an enzyme that in humans is encoded by the YES1 gene. This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. YES1 has been shown to interact with Janus kinase 2, CTNND1, RPL10 and Occludin.

Synonyms

Tyrosine-protein kinase Yes;2.7.10.2; Proto-oncogene c-Yes; p61-Yes; YES1; YES

Gene Name

YES1

Gene ID

7525

UniProt

P07947

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Yes1, different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis. .

Applications

IHC, WB

Field of Research

Cancer, Cancer Susceptibility, Epigenetics and Nuclear Signaling, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Proto-Oncogenes, Signal Transduction, Transcription, Tyrosine Kinases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. .

References & Citations

1. Entrez Gene: YES1 v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 2. Chen, Yan-Hua; Lu Qun; Goodenough Daniel A; Jeansonne Beverly (Apr 2002) .Nonreceptor tyrosine kinase c-Yes interacts with occludin during tight junction formation in canine kidney epithelial cells. Mol. Biol. Cell (United States) 13 (4) : 1227–37.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

60801 MW

Observed Molecular Weight

61 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Tyrosine-protein kinase Yes

Subcellular Location

Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytosol. Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway.

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Protein Name

Tyrosine-protein kinase Yes

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.