Anti-Actin/ACTA1 Antibody Picoband®

About this product

Boster Bio Anti-Actin/ACTA1 Antibody catalog # RP1070. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1070

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Synonyms

Actin, alpha skeletal muscle; Alpha-actin-1; ACTA1; ACTA

Gene Name

ACTA1

Gene ID

58

UniProt

P68133

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in cerebral cortex. .

Applications

IHC, WB

Field of Research

Actin, Actin Assembly, Actin Binding Proteins, Actin, etc., Apoptosis, Cancer, Cell Death, Cytoskeleton, Cytoskeleton/ECM, Invasion/Microenvironment, Microfilaments, Receptors, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

References & Citations

1. Gunning, P., Ponte, P., Okayama, H., Engel, J., Blau, H. and Kedes, L.Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.Mol. Cell. Biol.1983; 3 (5), 787-795. 2. Goebel, H.H., Brockmann, K., Bonnemann, C.G., Warlo, I.A., Hanefeld, F., Labeit, S., Durling, H.J. and Laing, N.G. Actin- related myopathy without any missense mutation in the ACTA1 Gene.J. Child Neurol.2004; 19 (2), 149-153. 3. Laing, N.G., Clarke, N.F., Dye, D.E., Liyanage, K., Walker, K.R., Kobayashi, Y., Shimakawa, S., Hagiwara, T., Ouvrier, R., Sparrow, J.C., Nishino, I., North, K.N. and Nonaka, I.Actin mutations are one cause of congenital fibre type disproportion.Ann. Neurol.2004; 56 (5), 689-694.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42051 MW

Observed Molecular Weight

42 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Actin, alpha skeletal muscle

Subcellular Location

Cytoplasm, cytoskeleton.

Sequence Similarities

Belongs to the synaptopodin family.

Protein Name

Actin, alpha skeletal muscle

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.