Anti-Aquaporin 4/AQP4 Antibody Picoband®

About this product

Boster Bio Anti-Aquaporin 4/AQP4 Antibody Picoband® catalog # PB9475. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9475

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.

Synonyms

Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4; AQP4

Gene Name

AQP4

Gene ID

361

UniProt

P55087

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Brain - muscle >> heart, kidney, lung, and trachea.

Applications

IHC, WB

Field of Research

Cancer, Channels, Metabolism, Plasma Membrane, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

References & Citations

1. Amiry-Moghaddam, M.; Otsuka, T.; Hurn, P. D.; Traystman, R. J.; Haug, F.-M.; Froehner, S. C.; Adams, M. E.; Neely, J. D.; Agre, P.; Ottersen, O. P.; Bhardwaj, A. : An alpha-syntrophin-dependent pool of AQP4 in astroglial end-feet confers biional water flow between blood and brain. Proc. Nat. Acad. Sci. 100: 2106-2111, 2003. 2. Agre P, Nielsen S (1996) . The aquaporin family of water channels in kidney. Nephrologie 17 (7) : 409–15. 3. Lennon, V. A.; Kryzer, T. J.; Pittock, S. J.; Verkman, A. S.; Hinson, S. R. : IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. J. Exp. Med. 202: 473-477, 2005. 4. Lu, M.; Lee, M. D.; Smith, B. L.; Jung, J. S.; Agre, P.; Verdijk, M. A. J.; Merkx, G.; Rijss, J. P. L.; Deen, P. M. T. : The human AQP4 gene: definition of the locus encoding two water channel polypeptides in brain. Proc. Nat. Acad. Sci. 93: 10908-10912, 1996. 5. Nagelhus EA, Mathiisen TM, Ottersen OP (2004) . Aquaporin-4 in the central nervous system: cellular and subcellular distribution and coexpression with KIR4.1. Neuroscience 129 (4) : 905–13.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34830 MW

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Aquaporin-4

Subcellular Location

Membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein Name

Aquaporin-4

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.