Anti-Aspartate beta hydroxylase/ASPH Antibody Picoband®

About this product

Boster Bio Anti-Aspartate beta hydroxylase/ASPH Antibody Picoband® catalog # PB9478. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9478

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ASPH is also known as Aspartyl/asparaginyl beta-hydroxylase. This gene is thought to play an important role in calcium homeostasis. And the gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF) -like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis.

Synonyms

Aspartyl/asparaginyl beta-hydroxylase;1.14.11.16 ; Aspartate beta-hydroxylase; ASP beta-hydroxylase; Peptide-aspartate beta-dioxygenase; ASPH; BAH

Gene Name

ASPH

Gene ID

444

UniProt

Q12797

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ASPH, identical to the related mouse sequence.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 is detected in all tissues tested. Isoform 8 is mainly expressed in pancreas, heart, brain, kidney and liver. Isoform 8 is expressed in kidney (at protein level) . .

Applications

Flow Cytometry, IHC, ICC, WB

Field of Research

Cell Biology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. .

References & Citations

1. Entrez Gene: ASPH aspartate beta-hydroxylase. 2. Korioth F, Gieffers C, Frey J (Feb 1995) . Cloning and characterization of the human gene encoding aspartyl beta-hydroxylase. Gene 150 (2) : 395–9. 3. Lim KY, Hong CS, Kim DH (Nov 2000) . cDNA cloning and characterization of human cardiac junctin. Gene 255 (1) : 35–42.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

85863 MW

Observed Molecular Weight

100 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Aspartyl/asparaginyl beta-hydroxylase

Subcellular Location

Isoform 1: Endoplasmic reticulum membrane; Single-pass type II membrane protein .

Sequence Similarities

Belongs to the aspartyl/asparaginyl beta-hydroxylase family.

Protein Name

Aspartyl/asparaginyl beta-hydroxylase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.