Anti-Cyclin A1/CCNA1 Antibody Picoband®

About this product

Boster Bio Anti-Cyclin A1/CCNA1 Antibody Picoband® catalog # PB9485. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9485

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cyclin-A1 is a protein that in humans is encoded by the CCNA1 gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. Also this cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Cyclin-A1; CCNA1

Gene Name

CCNA1

Gene ID

8900

UniProt

P78396

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Cyclin A1 recombinant protein (Position: D64-E263) . Human Cyclin A1 shares 78.5% and 79.5% amino acid (aa) sequence identity with mouse and rat Cyclin A1, respectively.

Clonality

Polyclonal

Tissue Specificity

Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Apoptosis, Cell Biology, Intracellular

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. .

References & Citations

1. Entrez Gene: CCNA1 cyclin A1. 2. Yang R, Morosetti R, Koeffler HP (March 1997) . Characterization of a second human cyclin A that is highly expressed in testis and in several leukemic cell lines.Cancer Res. 57 (5) : 913–20.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

52358 MW

Observed Molecular Weight

52 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cyclin-A1

Subcellular Location

Nucleus .

Sequence Similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Protein Name

Cyclin-A1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.