Anti-JAB1/COPS5 Antibody Picoband®

About this product

Boster Bio Anti-JAB1/COPS5 Antibody Picoband® catalog # PB9490. Tested in Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9490

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis), also known as COPS5 or JAB1, is a gene conserved from humans to Saccharomyces cerevisiae. It is a member of the MOV34 family. COPS5 is mapped to 8q13.1. The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. COPS5 can interact with the cytoplasmic domain of the beta-2 subunit of the alpha-L/beta-2 integrin LFA1, and it is the only protein demonstrated to interact with MIF. COPS5, VHL, and TRC8 proteins appear to be linked both physically and functionally, and all 3 may participate in the development of kidney cancer. In addition to that, COPS5 is an essential cofactor for the apoptotic function of E2F1.

Synonyms

COP9 signalosome complex subunit 5; SGN5; Signalosome subunit 5;3.4.-.-; Jun activation domain-binding protein 1; COPS5; CSN5, JAB1

Gene Name

COPS5

Gene ID

10987

UniProt

Q92905

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human JAB1 recombinant protein (Position: M8-S334) . Human JAB1 shares 99.4% amino acid (aa) sequence identity with mouse JAB1.

Clonality

Polyclonal

Tissue Specificity

Detected in platelets and in cell lines of megakaryocytic and erythrocytic lineages. Both isoform 1 and isoform 2 are detected in various cancer cell lines, with isoform 2 being the predominant form (at protein level) . Ubiquitously expressed. .

Applications

Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB

Field of Research

Proto-Oncogenes, SCF Complex E3 Ligase, Ser/Thr Kinases, Signal Transduction, Transcription, Ubiquitin & Ubiquitin Like Modifiers, Ubiquitin E3 Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts ly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. .

References & Citations

1. Bianchi, E., Denti, S., Granata, A., Bossi, G., Geginat, J., Villa, A., Rogge, L., Pardi, R.Integrin LFA-1 interacts with the transcriptional co-activator JAB1 to modulate AP-1 activity. Nature 404: 617-621, 2000. 2. Entrez Gene: COPS5 COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis) . 3. Hallstrom, T. C., Nevins, J. R. Jab1 is a specificity factor for E2F1-induced apoptosis.Genes Dev. 20: 613-623, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

38 kDa

Observed Molecular Weight

38 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

COP9 signalosome complex subunit 5

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Nuclear localization is diminished in the presence of IFIT3.

Sequence Similarities

Belongs to the peptidase M67A family. CSN5 subfamily.

Protein Name

COP9 signalosome complex subunit 5

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.