Anti-CPT1B Antibody Picoband®

About this product

Boster Bio Anti-CPT1B Antibody Picoband® catalog # PB9491. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9491

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CPT1B is located on 22q13.33. The protein encoded by this gene, a member of the carnitine/ choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene.

Synonyms

Carnitine O-palmitoyltransferase 1, muscle isoform; CPT1-M;2.3.1.21; Carnitine O-palmitoyltransferase I, muscle isoform; CPT I; CPTI-M; Carnitine palmitoyltransferase 1B; Carnitine palmitoyltransferase I-like protein; CPT1B; KIAA1670

Gene Name

CPT1B

Gene ID

1375

UniProt

Q92523

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CPT1B, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

Clonality

Polyclonal

Tissue Specificity

Strong expression in heart and skeletal muscle. No expression in liver and kidney.

Applications

IF, IHC, IHC-F, WB

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Obesity, Pathways and Processes, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts ly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. .

References & Citations

1. Auinger A; Rubin D; Sabandal M; Helwig U; Rüther A; Schreiber S; Foelsch UR; Döring F; Schrezenmeir J. “A common haplotype of carnitine palmitoyltransferase 1b is associated with the metabolic syndrome”. Br J Nutr, 2013 Mar 14.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

87801 MW

Observed Molecular Weight

88 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Carnitine O-palmitoyltransferase 1, muscle isoform

Subcellular Location

Mitochondrion outer membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the carnitine/choline acetyltransferase family.

Protein Name

Carnitine O-palmitoyltransferase 1, muscle isoform

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.